DNA Paternity Testing Electrophoresis Lab Activity

Code: 200-018

While over 99% of DNA is the same among all humans, the remaining part is unique to every individual (with the exception of identical twins). These differences are hereditary, with parts of the unique sequence coming from each parent.

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Description

While over 99% of DNA is the same among all humans, the remaining part is unique to every individual (with the exception of identical twins). These differences are hereditary, with parts of the unique sequence coming from each parent. By using DNA fingerprinting, or DNA profiling, a child’s paternal relationship can be tested by comparing the child’s DNA profile to the profile of the mother and possible father to determine if an individual is the father or not. In this activity students will use electrophoresis to separate DNA samples of a child, mother, and two potential fathers to determine if either father is a possible paternal match. The kit contains enough materials for 8 groups of students and includes a Teacher’s Guide and Student Study Guide copymasters. ( 200-018 )

Technical Specifications

Kit Includes:
200 mL Prepared Agarose 0.8% Solution
500 mL Tris Borate EDTA Buffer 5X
60 mL DNA Stain
10 μg Mother’s DNA Sample
10 μg Child’s DNA Sample
10 μg Possible Father #1 DNA Sample
10 μg Possible Father #2 DNA Sample

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